Forests by A & C Black Publishers Ltd

By A & C Black Publishers Ltd

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2010) by using a two-constructs approach. The first construct contained the FLP gene controlled by a heat-inducible promoter as well as a marker gene under control of the 35S promoter, while the second carried a promoterless second marker gene. Poplar plants were double transformed with the two constructs, and following heat induction of FLP, the promoterless second marker gene became active by exactly replacing the FLP gene/first marker gene and getting under control of the 35S promoter (Fladung et al.

The functionality of transposons was first shown by Fladung and Ahuja (1997) and Kumar and Fladung (2003). Later, mobility of transposons across chromosomal barriers was confirmed by Fladung (2011) and Fladung and Polak (2012). A relocated transposon-borne transgene is free from unwanted T-DNA sequences and may be less prone to gene silencing than at the original integration site. Such a method could be useful for quickly generating large populations of T-DNA site-free transgenic plants. , will also increase the need for food production.

These systems are the most commonly used in plant biotechnology (Puchta 2003). Because of the identical nature of the recognition sites the recombination reaction is fully reversible, although intra-molecular recombination (excision) is highly favored over inter-molecular reactions (integration). The unidirectional tyrosine subfamily has nonidentical recognition sites typically known as attB (attachment site bacteria) and attP (attachment site phage) and performs irreversible recombination: HK022 (Gottfried et al.

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