By Ado Jorio
This booklet covers the improvement of biotechnology in keeping with carbon nanostructures, with a spotlight on nanotubes, addressing additionally fullerenes and amorphous carbons. The publication is split into 7 chapters, addressing tissue engineering, genetic engineering and remedy, in addition to the environmental and wellbeing and fitness affects of carbon nanostructures.
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De Mello Brandão et al. Fig. 3 The fluorescence of bovine fibroblast cells successfully transfected with GFP gene: a MWCNT-COOH with GFP gene; b poly(ethyleneimine) with GFP gene [99] the amount of gene or protein conjugated by MWCNT-CHOO is still limited, resulting in low transfection efficiency (Fig. 3a) when compared to the reagent poly (ethyleneimine) (Fig. 3b). 2 Contribution of CNTs to Conventional Techniques In somatic cells, embryonic-stem cells or in sperm-mediated gene transfer, several methods have been developed for the establishment of an efficient gene delivery system, such as viral vectors [100–102] or nonviral ones, like liposomes [5, 103], physical or chemical methods [104–106].
3a). However, 42 H. de Mello Brandão et al. Fig. 3 The fluorescence of bovine fibroblast cells successfully transfected with GFP gene: a MWCNT-COOH with GFP gene; b poly(ethyleneimine) with GFP gene [99] the amount of gene or protein conjugated by MWCNT-CHOO is still limited, resulting in low transfection efficiency (Fig. 3a) when compared to the reagent poly (ethyleneimine) (Fig. 3b). 2 Contribution of CNTs to Conventional Techniques In somatic cells, embryonic-stem cells or in sperm-mediated gene transfer, several methods have been developed for the establishment of an efficient gene delivery system, such as viral vectors [100–102] or nonviral ones, like liposomes [5, 103], physical or chemical methods [104–106].
3a). However, 42 H. de Mello Brandão et al. Fig. 3 The fluorescence of bovine fibroblast cells successfully transfected with GFP gene: a MWCNT-COOH with GFP gene; b poly(ethyleneimine) with GFP gene [99] the amount of gene or protein conjugated by MWCNT-CHOO is still limited, resulting in low transfection efficiency (Fig. 3a) when compared to the reagent poly (ethyleneimine) (Fig. 3b). 2 Contribution of CNTs to Conventional Techniques In somatic cells, embryonic-stem cells or in sperm-mediated gene transfer, several methods have been developed for the establishment of an efficient gene delivery system, such as viral vectors [100–102] or nonviral ones, like liposomes [5, 103], physical or chemical methods [104–106].