By C. A. Maynard, K. Kavanagh, H. Fuernkranz, A. P. Drew (auth.), Professor Dr. Y. P. S. Bajaj (eds.)
After the 1986 and 1989 volumes, this is often the 3rd quantity on biotechnology for propagation of timber. Comprising 28 chapters contributed through foreign specialists the publication offers with fruit, decorative, and woodland timber, equivalent to Black cherry, bitter cherry, Pomegranate, Loquat, Ficus, Yellow poplar, Horse chestnut, Judas tree, Linden tree, Saskatoons, Taiwan sassafras, Plane-tree, Rattans, Bamboos, Engelmann spruce, White spruce, Larches, Hinoki cypress, Western redcedar, and diverse sorts of pines, i.e. Jack, Carribean, Eldarica, reduce, Egg-cone, Maritime, Ponderosa, jap white, Loblolly pine. Trees III is a superb reference publication for scientists, educators, and scholars of forestry, botany, genetics, and horticulture, who're drawn to tree biotechnology.
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Gella cultures established from stem pieces with lateral buds usually developed bacterial contamination after several months of culture, forming white clouds at the base of the shoots. Erwinia sp. is commonly present in these colonies (Robert pers. ). On the other hand, smaller explants grew more slowly and survival and shoot growth were smaller. From a total of 98 dissected lateral buds taken in summer, about 21% did not grow into shoots, but developed callus tissue. 2 Sterilization Surface contaminants are eliminated by treating the explants with some disinfectant solution.
Explant size is not as important for micropropagation purposes as for obtaining virus-free plants. However, the smaller the explant, the less the possibility of including germs which could develop in culture, even several months after establishment. In our laboratory, cultures of P. cerasus rootstock clones established from lateral buds and dissected up to about 2 mm by removing the outer scales (Fig. 5-2 cm long. Steriliza tion. Isolation of 1-2·mm shoot tips Shoot tips and stem nodes taken in May.
McGraw-Hill, New York Peterson TA (1988) Wisconsin Forest products price review, May 1988. Coop Extens Serv, Univ Wisconsin Pitcher JA (1982) Phenotypic selection and half-sib family performance in black cherry. For Sci 28:251-256 Pitcher JA (1984) Geographic variation patterns in seed and nursery characteristics of black cherry. USDA For Serv S For Exp Stn Res Pap SO-208 Quoirin MP, Lepoivre P, Boxus P (1977) Un premier bilan de 10 annees de recherches sur les cultures de meristemes et la multiplication in vitro de fruitiers ligneux.