By Eng-Chong Pua, Michael R. Davey (eds.)
Genetic engineering is a robust software for crop development. The prestige of crop biotechnology prior to 2001 was once reviewed in Transgenic vegetation I-III, yet fresh advances in plant telephone and molecular biology have triggered the necessity for brand new volumes.
Following Transgenic plants IV (2007) facing cereals, greens, root vegetation, herbs and spices, this quantity, Transgenic vegetation V, is dedicated to fruit, timber and beverage vegetation. Fruit vegetation special comprise banana, citrus, mango, papaya, pineapple, watermelon, avocado, grape, melon, apple, Prunus spp., strawberry, and kiwifruit, whereas bushes contain walnut, rubber, eucalyptus, tropical tree legumes, Casuarina glauca and conifers. The part on beverage vegetation studies reports on espresso, cacao, tea, and sugarcane.
This quantity is a useful reference for plant breeders, researchers and graduate scholars within the fields of plant biotechnology, agronomy, horticulture, forestry, genetics, and either plant cellphone and molecular biology.
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Extra resources for Transgenic Crops V
Example text
SSE grown on MS with 5 mg l−1 BA developed into mature embryos that further developed into plants after being transferred to hormone-free MS. Explants formed embryogenic callus on M1 medium with 18 μM 2,4-D for 3 months, followed by 2–3 months on the same medium with 9 μM 2,4-D. 8 μM BA, callus formed SEs, which developed into adventitious shoots in the dark. Shoots formed roots on hormone-free MS medium. 3 μM NAA and 6 g l−1 agarose formed androgenic callus. 5 g l−1 agarose. b. cv. Pisang Klutuk, Pisang Klutuk Wulung, Pisang Batu and Tani (BB) Musa species/ cultivar (group)a Table 1.
Continued) Escalant et al. (1994) Grapin et al. (1996) Vuylsteke and Ortiz (1996) Reference Protoplasts isolated from cell suspension were grown in half MS medium Panis et al. (1993) with 5 μM 2,4-D, 5% mannitol and 2 g l−1 Gelrite with nurse culture (cell suspension). While 50% of protoplasts showed initial cell division, 20–40% formed microcalli. Microcalli were transferred to hormone-free medium, where they developed SEs and plants after 3 months. Explants formed callus on MM1 medium after 3 months.
The regeneration protocols via SE reported for various banana genotypes are quite similar (see Table 1). In general, explants are cultured on semi-solid MS-based medium supplemented with auxins to induce formation of embryogenic callus, which is used to initiate cell suspensions. Embryogenic cells are then induced to differentiate into embryos in an appropriate medium containing a combination of auxin and cytokinin. Embryos germinate and develop into plants after transfer to hormone-free semi-solid medium.